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Detection of periodontal disease related bacteria from the implant-abutment interface in oral cavity

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ÇѸíÁÖ ( Han Myong-Joo ) - Á¶¼±´ëÇб³ Ä¡°ú´ëÇÐ º¸Ã¶Çб³½Ç
À¯¼Ò¿µ ( Yoo So-Young ) - Á¶¼±´ëÇб³ Ä¡°ú´ëÇÐ ±¸°­»ýÈ­Çб³½Ç
±¹Áß±â ( Kook Joong-Ki ) - Á¶¼±´ëÇб³ Ä¡°ú´ëÇÐ ±¸°­»ýÈ­Çб³½Ç
±èÈñÁß ( Kim Hee-Joong ) - Á¶¼±´ëÇб³ Ä¡°ú´ëÇÐ º¸Ã¶Çб³½Ç
Á¤ÀçÇå ( Chung Chae-Heon ) - Á¶¼±´ëÇб³ Ä¡°ú´ëÇÐ º¸Ã¶Çб³½Ç

Abstract

¹®Á¦ Áø¼ú: ÀÓÇ÷£Æ® ½Ã½ºÅÛÀº ÀÓÇ÷£Æ®¿Í Áö´ëÁÖ »çÀÌ¿¡¼­ ºó °ø°£ÀÌ ¹ß»ýÇÏ¿© ¼¼±ÕÀÇ ÀúÀå¼Ò·Î¼­ ÀÛ¿ëÇÏ°Ô µÇ¾î ÀÓÇ÷£Æ® ÁÖÀ§ ¿¬Á¶Á÷ ¿¡ ¿°Áõ¹ÝÀÀÀ» ¾ß±âÇÒ ¼ö ÀÖ´Ù.

¸ñÀû: ÀÌ ¿¬±¸ÀÇ ¸ñÀûÀº ÀÓÇ÷£Æ®-Áö´ëÁÖ °£±ØÀÇ ¹Ì¼¼ ´©Ãâ¿¡ °ü¿©ÇÏ´Â Ä¡ÁÖÁúȯ¿øÀαÕÀ¸·Î ÃßÁ¤µÇ´Â Porpyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, Treponema denticola, Aggregatibacter actinomycetemcomitansÀÇ °ËÃâ·üÀ» Á¶ »çÇÏ´Â °ÍÀÌ´Ù.

¿¬±¸Àç·á ¹× ¹æ¹ý: ½Ã·áäÃë´Â 27¸íÀÇ È¯Àڵ鿡¼­ ÇàÇÏ¿´°í ¼Òµ¶µÈ ÆäÀÌÆÛÆ÷ÀÎÆ®¸¦ ÀÌ¿ëÇÏ¿© 1¡¿PBS·Î ¿Å°å´Ù. Ä¡ÁÖÁúȯ¿øÀαÕÀÇ °ËÃâ Àº 16 rDNA¿¡ ±Ù°ÅÇÑ Á¾Æ¯ÀÌÀûÀÎ ÇÁ¶óÀ̸Ӹ¦ Áö´Ñ ÁßÇÕÈ¿¼ÒüÀιÝÀÀÀ» ÀÌ¿ëÇÏ¿´´Ù.

°á°ú: ÀÓÇ÷£Æ® °íÁ¤Ã¼³»¿¡¼­ÀÇ Porphyromonas gingivalis¿Í Prevotella intermediaÀÇ °ËÃâ·üÀº 59%¿Í 82%¿´´Ù. ȯÀÚÀÇ ÀÓÇà ·£Æ® ¿¬±¸¿¡¼­´Â Porphyromonas gingivalis¿Í Prevotella intermediaÀÇ °ËÃâ·üÀº 44%¿Í 82%¿´´Ù. ȯÀÚÇô¿¡¼­ÀÇ Porphyromonas gingivalis¿Í Prevotella intermediaÀÇ °ËÃâ·üÀº 82%¿Í 82%¿´´Ù.

°á·Ð: ÇöÀçÀÇ ÀÓÇ÷£Æ® ½Ã½ºÅÛÀº ÀÓÇ÷£Æ® ³»ºÎÀÇ ¼¼±Õ ±ºÁýÈ­¿Í ¹Ì¼¼´©ÃâÀ» ¾ÈÀüÇÏ°Ô ¿¹¹æÇÒ ¼ö ¾ø´Ù.

Statement of the problem: Implant systems result in gaps and cavities between implant and abutment that can act as a trap for bacteria and thus possibly cause inflammatory reactions in the peri-implant soft tissues.

Purpose: Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, Treponema denticola, and Aggregatibacter actinomycetemcomitans, related to implant-abutment interface microleakage.

Material and methods: Samples were taken from 27 subjects with sterilized paper points and were transported in 1¡¿PBS. The detection of periodontopathogens were performed by polymerase chain reaction with species-specific primers based on 16S rDNA.

Results: Our data showed that the detection rate of P. gingivalis and P. intermedia in implant fixture was 59% and 82% in patients respectively. Detection rate of P. gingivalis and P. intermedia in implant crevice was 44% and 82% in patients. Detection rate of P. gingivalis and P. intermedias in tongue was 82% and 82% in patients.

Conclusion: Current implant systems cannot safely prevent microbial leakage and bacterial colonization of the inner part of the implant.

Å°¿öµå

ÀÓÇ÷£Æ®-Áö´ëÁÖ ¿¬°áºÎ;Ä¡ÁÖÁúȯ ¿øÀαÕ;¹Ì¼¼´©Ãâ;Porphyromonas gingivalis
Implant-abutment interface;Periodontopathogens;Microbial leakage;Porphyromonas gingivalis

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